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11:28 min
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October 01, 2014
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Summary
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In this video protocol we track - at high speed and in three dimensions - fluorescently labeled lysosomes within living cells, using the orbital tracking method in a modified two-photon microscope.
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Cite this Article
Anzalone, A., Annibale, P., Gratton, E. 3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles. J. Vis. Exp. (92), e51794, doi:10.3791/51794 (2014).
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