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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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Bioengineering
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JoVE Journal Bioengineering
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

DOI:
10.3791/51794-v

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11:28 min

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October 01, 2014

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, ,
1Biomedical Engineering, Laboratory for Fluorescence Dynamics,University of California, Irvine

Chapters

  • 00:05Title
  • 01:50Sample Preparation
  • 03:19Microscope Configurations
  • 05:05Imaging
  • 07:21Trajectory Analysis
  • 08:40Results: Fast 3D Single Particle Tracking of an Isolated Lysosome Moving Inside the Cell After the Endosome Maturation Process
  • 10:36Conclusion

Summary

Automatic Translation

Automatic Translation

In this video protocol we track - at high speed and in three dimensions - fluorescently labeled lysosomes within living cells, using the orbital tracking method in a modified two-photon microscope.

Tags

Keywords: 3D Orbital TrackingTwo-photon MicroscopyParticle TrackingLysosome TrackingIntracellular Vesicle TrackingHigh-resolution TrackingFeedback AlgorithmLive Cell ImagingMicrotubule Dynamics

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