14,234 Views
•
12:00 min
•
July 08, 2014
DOI:
Summary
Automatically generated
This article describes a neuronal culture system that can be used to obtain large quantities of pure axonal samples for biochemical and immunocytochemical analyses. This technique will allow an improved understanding of normal axonal physiology and signaling pathways leading to neurodegeneration.
Related Videos
05:29
Axoplasm Isolation from Rat Sciatic Nerve
Related Videos
13637 Views
08:30
Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
Related Videos
31550 Views
09:42
Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
Related Videos
26651 Views
09:40
Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
Related Videos
12255 Views
11:34
Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels
Related Videos
6716 Views
08:06
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
Related Videos
6041 Views
10:12
Designing Porous Silicon Films as Carriers of Nerve Growth Factor
Related Videos
9743 Views
08:16
Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
Related Videos
7188 Views
06:55
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
Related Videos
1790 Views
01:15
Isolation of Cells from Sensory Ganglia
Related Videos
93 Views
Read Article
Cite this Article
Unsain, N., Heard, K. N., Higgins, J. M., Barker, P. A. Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters. J. Vis. Exp. (89), e51795, doi:10.3791/51795 (2014).
Copy