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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Bioengineering
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JoVE Journal Bioengineering
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

DOI:
10.3791/51797-v

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14:53 min

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September 10, 2014

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, , , ,
1MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research,University of Maryland, 2Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering,University of Maryland

Chapters

  • 00:05Title
  • 02:06Microfabrication of the Electrochemical Chip
  • 06:36Fabrication of the PDMS Chip
  • 08:52Assembly and Preparation of the Microfluidic Arrayed Electrochemical Device
  • 10:44DNA Hybridization Analysis
  • 12:14Validating Electrochemical Activity of the Biochip and Detection of Hybridization Events
  • 13:55Conclusion

Summary

Automatic Translation

Automatic Translation

We present a microfluidic-based electrochemical biochip for DNA hybridization detection. Following ssDNA probe functionalization, the specificity, sensitivity, and detection limit are studied with complementary and non-complementary ssDNA targets. Results illustrate the influence of the DNA hybridization events on the electrochemical system, with a detection limit of 3.8 nM.

Tags

Keywords: MicrofluidicElectrochemical BiochipLabel-freeDNA HybridizationAnalysisMiniaturizationPoint-of-careElectrochemical Impedance SpectroscopyCharge TransferDiffusional ResistanceBiosensorSsDNA Detection

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