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JoVE Journal Bioengineering
Un Biochip elettrochimica Microfluidic-based per la libera-Label Ibridazione DNA Analysis
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Un Biochip elettrochimica Microfluidic-based per la libera-Label Ibridazione DNA Analysis

Article DOI: 10.3791/51797-v • 14:53 min • September 10th, 2014
September 10th, 2014 •
1, 1, 2, 2, 1
1MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, 2Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering, University of Maryland

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Vi presentiamo un biochip elettrochimico basato microfluidica-per la rilevazione ibridazione DNA. A seguito di ssDNA sonda funzionalizzazione, la specificità, sensibilità, e il limite di rilevazione sono studiati con obiettivi ssDNA complementari e non complementari. I risultati illustrano l'influenza degli eventi di ibridazione DNA sul sistema elettrochimico, con un limite di rilevazione di 3,8 nM.

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Bioingegneria spettroscopia di impedenza elettrochimica ibridazione DNA biosensori biochip microfluidica la rilevazione senza etichetta diffusione ristretta microfabrication
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