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Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

DOI:
10.3791/51826-v

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11:42 min

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September 12, 2014

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, , , , , , ,
1Department of Materials Science and Engineering,University of Sheffield, 2Department of Chemistry,University of Sheffield, 3L. V. Prasad Eye Institute

Chapters

  • 00:05Title
  • 02:15Fabrication of PEGDA Templates by Microstereolithography
  • 03:58Fabrication of Biodegradable PLGA Membranes Using Electrospinning
  • 05:48Long-term Storage of PLGA Microfabricated Membranes
  • 06:34Isolation of Limbal Explants
  • 07:53Inclusion of Ring Scaffolds in Rabbit Cornea 3D Models
  • 08:46Results: Synthetic Microstructured Membranes Support Corneal Regeneration
  • 10:48Conclusion

Summary

Automatic Translation

Automatic Translation

We report a technique for the fabrication of micropockets within electrospun membranes in which to study cell behavior. Specifically, we describe a combination of microstereolithography and electrospinning for the production of PLGA (Poly(lactide-co-glycolide)) corneal biomaterial devices equipped with microfeatures.

Tags

Keywords: Corneal RegenerationMicrostereolithographyElectrospinningCorneal MembranesLimbal Stem CellsPalisades Of VogtCorneal DiseaseCorneal GraftsStem Cell CarriersCorneal Biomaterial DevicesPlatform Technology

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