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JoVE Journal Chemistry
Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
 

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Article doi: 10.3791/51840
November 20th, 2014 •
1,2, 1, 1,3, 1,2, 1
1Complex Carbohydrate Research Center, University of Georgia, 2Department of Biochemistry and Molecular Biology, University of Georgia, 3Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

Summary
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November 20th, 2014

In order to comprehensively explore the diversity of O-linked glycans, a new procedure for in-gel reductive β-elimination, combined with permethylation and a rapid phase-partition method, is applied to the analysis of O-linked glycans directly released from glycoproteins resolved by SDS-PAGE and amenable to subsequent glycomic analysis by mass spectrometry.

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