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JoVE Journal Bioengineering
Utilization of Microscale Silicon Cantilevers to Assess Cellular Contractile Function In Vitro
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Utilization of Microscale Silicon Cantilevers to Assess Cellular Contractile Function In Vitro

Article DOI: 10.3791/51866-v • 10:53 min • October 3rd, 2014
October 3rd, 2014 •
1, 1, 1, 1, 1, 1
1NanoScience Technology Center, University of Central Florida

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This protocol describes the use of microscale silicon cantilevers as pliable culture surfaces for measuring the contractility of muscle cells in vitro. Cellular contraction causes cantilever bending, which can be measured, recorded, and converted into readouts of force, providing a non-invasive and scalable system for measuring contractile function in vitro.

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Microscale Silicon Cantilevers Cellular Contractile Function In Vitro Assays Cell Culture Systems Functional Assessment Microscale Cantilever Technology Contraction-induced Substrate Bending Multiplexed Cantilever Arrays Drug Efficacy And Toxicity Disease Phenotype And Progression Neuromuscular Interactions Fabricating Reliable Cantilever Arrays Cell Culture On Cantilever Surfaces Functional Analysis Of Contractile Cell Types Laser And Photo-detector System Precision And Reproducibility Of Analysis Wide Range Of Applications
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