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Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents
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Behavior
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JoVE Journal Behavior
Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents

Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents

DOI:
10.3791/51869-v

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14:02 min

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June 29, 2014

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, , , , , , , , , , , , , ,
1Neuroscience Center,University of Helsinki, 2Neurotar LTD, 3A. I. Virtanen Institute for Molecular Sciences,University of Eastern Finland, 4Laboratory Animal Center,University of Helsinki

Chapters

  • 00:00Title
  • 01:12Cranial Window Implantation
  • 01:33Training
  • 05:35Applications: Two-photon Microscopy Imaging
  • 08:41Applications: Intrinsic Optical Imaging
  • 09:59Applications: Electrophysiology
  • 10:54Behavioral Experiments
  • 11:08a) Habituation-dishabituation Olfactory Test
  • 12:17b) Reaction to Novelty
  • 13:03Conclusions

Summary

Automatic Translation

Automatic Translation

This method creates a tangible, familiar environment for the mouse to navigate and explore during microscopic imaging or single-cell electrophysiological recordings, which require firm fixation of the animal’s head.

Tags

Keywords: Flat-floored Air-lifted PlatformAwake Freely Moving RodentsNon-anesthetized Behaving MiceCellular And Cognitive NeurosciencesHead-fixed MouseTwo-photon Microscopic ImagingPatch-clamp RecordingsBehavioral TestsLearningHabituationNovel Object Recognition

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