Journal
/
Immunology and Infection
/
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

DOI:
10.3791/51894-v

•

06:40 min

•

September 09, 2014

•

, , ,
1Department of Pathology,Northwestern University

Chapters

  • 00:05Title
  • 01:21Purification of Fetal Liver Erythroblasts
  • 02:32Purification of TER119 Negative Erythroblasts
  • 03:50Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture
  • 04:57Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

We present an in vitro mouse fetal liver erythroblast culture system that dissects the early and late stages of terminal erythropoiesis. This system facilitates functional analysis of specific genes in different developmental stages.

Tags

Mouse Fetal LiverErythropoiesisErythroid Burst Forming Units (BFU-E)Erythroid Colony Forming Units (CFU-E)Terminal ErythroblastsTER119Erythroid Progenitor CellsGene Function AnalysisEarly And Late Stages Of Terminal ErythropoiesisCDNA OverexpressionShRNA KnockdownErythropoietin (Epo)

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51894?article_type=v

Introducing Shear Stress in the Study of Bacterial Adhesion
check_url/51894?article_type=v

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model
check_url/51894?article_type=v

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
check_url/51894?article_type=v

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
check_url/51894?article_type=v

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
check_url/51894?article_type=v

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
check_url/51894?article_type=v

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
check_url/51894?article_type=v

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
check_url/51894?article_type=v

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
check_url/51894?article_type=v

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Read Article