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JoVE Journal Neuroscience
Preparação do Synaptic Plasma Membrane e Proteínas de densidade pós-sinápticos Usando um gradiente de sacarose descontínuo
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Preparação do Synaptic Plasma Membrane e Proteínas de densidade pós-sinápticos Usando um gradiente de sacarose descontínuo

Article DOI: 10.3791/51896-v • 08:06 min • September 3rd, 2014
September 3rd, 2014 •
*1, *1, 1, 1
1Department of Pharmacology and Toxicology, University of Toronto
* These authors contributed equally

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Este artigo fornece detalhes sobre o enriquecimento de proteínas associadas com a membrana de plasma sináptico por ultracentrifugação num gradiente de sacarose descontínuo. A elaboração definitiva de proteínas de densidade pós-sináptica é também descrito. Preparações de proteínas são adequados para western blot ou análise 2D DIGE.

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Neurobiologia cérebro sinapse western blot ultracentrifugação SPM PSD
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