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Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia
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Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia

DOI:
10.3791/51900-v

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10:24 min

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November 17, 2014

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, , , , , , , , ,
1Department of Plastic Surgery and Hand Surgery, Klinikum rechts der Isar,Technische Universität München, 2Department of Plastic, Reconstructive, Aesthetic and Hand Surgery,University Hospital of Basel, 3Institute for Clinical and Experimental Surgery,University of Saarland, 4Division of Plastic and Hand Surgery,University Hospital Zurich

Chapters

  • 00:05Title
  • 01:35Animal Preparation and Surgical Elevation of the Flap
  • 06:09Intravital Epifluorescence Microscopy
  • 07:57Results: Tissue Remodeling in Response to Acute Persistent Ischemia
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

The window of the murine dorsal skinfold chamber presented visualizes a zone of acute persistent ischemia of a musculocutaneous flap. Intravital epi-fluorescence microscopy permits for direct and repetitive assessment of the microvasculature and quantification of hemodynamics. Morphologic and hemodynamic results can further be correlated with histological and molecular analyses.

Tags

Dorsal Skinfold ChamberMouse Skin Flap ModelAcute Persistent IschemiaMicrovascular ArchitectureHemodynamicsIntravital Epi-fluorescence MicroscopyApoptosisInflammationMicrovascular LeakageAngiogenesisPreconditioningPericonditioningPostconditioning

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