Journal
/
Medicine
/
Real-time Cytotoxicity Assays in Human Whole Blood
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Real-time Cytotoxicity Assays in Human Whole Blood

Real-time Cytotoxicity Assays in Human Whole Blood

DOI:
10.3791/51941-v

•

08:27 min

•

November 07, 2014

•

, , ,
1Research and Development,Adheren, Inc, 2Research and Development,Eureka Therapeutics

Chapters

  • 00:05Title
  • 01:05Experimental Setup and Target Cell Preparation
  • 02:46Preparation of Cell Microarrays
  • 04:05Whole Blood Cytotoxicity Assays
  • 06:03Measuring Raji Cell Death via WCA
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

The whole blood cytotoxicity assay (WCA) is a cytotoxicity assay developed by incorporating high-throughput cell positioning technology with fluorescence microscopy and automated image processing. Here, we describe how lymphoma cells treated with an anti-CD20 antibody can be analyzed real-time in human whole blood to provide quantitative cellular cytotoxicity analysis.

Tags

Keywords: Real-time Cytotoxicity AssayHuman Whole BloodCell-based AssayCell CytotoxicityTumor Cell PopulationsAntibody DrugsCell DeathCell KillingCell SurvivalTime-dependent CytotoxicityDose-dependent CytotoxicityLymphomaLeukemiaSolid TumorEx Vivo

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51941?article_type=v

Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
check_url/51941?article_type=v

Real-time fMRI Biofeedback Targeting the Orbitofrontal Cortex for Contamination Anxiety
check_url/51941?article_type=v

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood
check_url/51941?article_type=v

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
check_url/51941?article_type=v

Movement Retraining using Real-time Feedback of Performance
check_url/51941?article_type=v

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
check_url/51941?article_type=v

Chemotherapy-induced Vascular Toxicity – Real-time In vivo Imaging of Vessel Impairment
check_url/51941?article_type=v

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
check_url/51941?article_type=v

Real-time X-ray Imaging of Lung Fluid Volumes in Neonatal Mouse Lung
check_url/51941?article_type=v

Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR

Read Article