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Journal / Immunology and Infection / Propagation d'
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Immunology and Infection

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Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
Journal (Immunology and Infection)
Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Journal (Immunology and Infection)
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
 
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Propagation d'

Article DOI: 10.3791/51953-v • 12:52 min • September 25th, 2014
September 25th, 2014 •
1, 1, 2, 1
1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS

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Nous présentons ici un protocole de propager les cellules de Homalodisca et HoCV-1 in vitro. Le milieu a été retiré de HoCV-1 et des cultures positives ARN extrait tous les 24 h pendant 168 h. Cellule de survie a été quantifiée par coloration au bleu trypan. Particules virales entières ont été extraits après l'infection. L'ARN extrait a été quantifiée par qRT-PCR.

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Infection Numéro 91, La culture cellulaire la maladie de Pierce de la vigne,
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