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Analysis of Cell Migration within a Three-dimensional Collagen Matrix
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Bioengineering
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JoVE Journal Bioengineering
Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

DOI:
10.3791/51963-v

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08:02 min

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October 05, 2014

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, , , ,
1Institute of Immunology & Experimental Oncology, Center for Biomedical Education and Research (ZBAF),Witten/Herdecke University

Chapters

  • 00:05Title
  • 01:09Collagen Suspension Cell Mix in Migration Chambers
  • 02:55Recording and Analysis of Cell Migration
  • 04:07Data Analysis
  • 05:09Results: Migratory Phenotype of Breast Cancer Cells
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

Cell migration is a biological phenomenon that is involved in a plethora of physiological, such as wound healing and immune responses, and pathophysiological processes, like cancer. The 3D-collagen matrix migration assay is a versatile tool to analyze the migratory properties of different cell types within in a 3D physiological-like environment.

Tags

Keywords: Cell Migration3D Collagen MatrixExtracellular MatrixCell LocomotionTime-lapse Video MicroscopyCell TypesCell ClustersSpheroidsMetastasisWound HealingImmune ResponseEmbryonic Development

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