Name: Dissection of Drosophila Ovaries (Video) | JoVE Journal
Uploaded: 2006-10-19T00:00:00.000+00:00
Duration: 4 min 55 s
Description: Learn the dissection of Drosophila ovaries for developmental biology research using Drosophila melanogaster. This technique enables precise examination of germline development and oogenesis.

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5 minutes

Protocol for Drosophila ovary dissection
<ol>
<li>Feed flies with yeast 1-2 days prior to dissecting them to fatten up the ovaries.</li>
<li>Anaesthetize flies using carbon dioxide stream.</li>
<li>Using a pair of tweezers, select a female fly.</li>
<li>Submerge the female fly into 1X PBS.</li>
<li>Grab the fly at its lower thorax with a pair of tweezers.</li>
<li>Tug gently at the lower abdomen with another pair of tweezers until the internal organs in the abdomen are exposed.</li>
<li>Look for the pair of ovaries and detach it from other organs (e.g. the intestines). </li>
<li>Tease apart the ovarioles (if ovaries are to be used for immunostaining or in situ hybridization). </li>
<li>Keep ovaries in ice-cold 1X PBS while dissecting the next fly.</li>
</ol>

<ol><li>Costa, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+Drosophila+fragile+X+protein+functions+as+a+negative+regulator+in+the+orb+autoregulatory+pathway%5BTitle%5D)+AND+%22Dev+Cell%22%5BJournal%5D)">The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway.</a> Dev Cell. 8, 331-342 (2005).</li>
<li>Tan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+autoregulatory+feedback+loop+directs+the+localized+expression+of+the+Drosophila+CPEB+protein+Orb+in+the+developing+oocyte%5BTitle%5D)+AND+%22Development%22%5BJournal%5D)">An autoregulatory feedback loop directs the localized expression of the Drosophila CPEB protein Orb in the developing oocyte.</a> Development. 128, 1159-1169 (2001).</li>
</ol>

Here we demonstrate a method of dissecting ovaries in Drosophila. As the method of culturing the different cell types that exist in the Drosophila ovary has yet been defined, this is a rapid method to obtain the Drosophila ovarian tissue. Essentially, we show that dissecting Drosophila ovaries involves a simple two- or three-step procedure. Subsequent treatment of these dissected ovaries depend on the downstream experiments that will be performed.

<table><tbody><tr><td>Forceps</td><td>Tool</td><td>Sigma-Aldrich</td><td>T43537</td><td></td></tr><tr><td>Glass dissecting dish</td><td>Tool</td><td>Pyrex</td><td>7220-85</td><td></td></tr></tbody></table>

dissection of drosophila ovaries

Dissection of Drosophila Ovaries

Research

JoVE Journal

Biology

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Dissection of Oenocytes from Adult Drosophila melanogaster

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The basement membrane is essential for tissue and organ morphogenesis during development. To better understand the mechanisms leading to proper placement of this structure, the protocol presented describes methods to visualize and characterize the intracellular trafficking and secretion of basement membrane proteins in epithelial cells using confocal and super-resolution microscopy.

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Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches

Learn the dissection of Drosophila ovaries for developmental biology research using Drosophila melanogaster. This technique enables precise examination of germline development and oogenesis.

Dissection of Drosophila Ovaries

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