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JoVE Journal
Developmental Biology

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Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies
 

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

Article doi: 10.3791/52002
November 2nd, 2015

Summary November 2nd, 2015

Embryonic chick retinal cell cultures constitute valuable tools for the study of photoreceptor biology. We have developed an efficient gene transfer technique based on ex ovo plasmid electroporation of the retinas prior to culture. This technique considerably increases transfection efficiencies over currently available protocols, making genetic manipulation feasible for this system.

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