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Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
JoVE Journal
Biology
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JoVE Journal Biology
Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

10,678 Views

07:47 min

November 06, 2014

DOI:

07:47 min
November 06, 2014

10657 Views
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Summary

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Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker.

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