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07:47 min
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November 06, 2014
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Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker.
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Cite this Article
Cohen, I., Geffen, Y., Ravid, G., Ravid, T. Reporter-based Growth Assay for Systematic Analysis of Protein Degradation. J. Vis. Exp. (93), e52021, doi:10.3791/52021 (2014).
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