Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker.
Cohen, I., Geffen, Y., Ravid, G., Ravid, T. Reporter-based Growth Assay for Systematic Analysis of Protein Degradation. J. Vis. Exp. (93), e52021, doi:10.3791/52021 (2014).