Here, a novel quantitative fluorescence assay is developed to measure changes in the level of a protein specifically at centrosomes by normalizing that protein’s fluorescence intensity to that of an appropriate internal standard.
Majumder, S., Fisk, H. A. Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes. J. Vis. Exp. (94), e52030, doi:10.3791/52030 (2014).