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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
JoVE Journal
Biology
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JoVE Journal Biology
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

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09:39 min

December 20, 2014

DOI:

09:39 min
December 20, 2014

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Summary

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Here, a novel quantitative fluorescence assay is developed to measure changes in the level of a protein specifically at centrosomes by normalizing that protein’s fluorescence intensity to that of an appropriate internal standard.

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