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Biology

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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
 

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Article DOI: 10.3791/52030
December 20th, 2014

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Summary December 20th, 2014

Here, a novel quantitative fluorescence assay is developed to measure changes in the level of a protein specifically at centrosomes by normalizing that protein’s fluorescence intensity to that of an appropriate internal standard.

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