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マラリア蚊の開発、産卵および生存者にエピジェネティック薬の試験に及ぼす影響のために毒物学的アッセイ
JoVE Journal
Biology
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JoVE Journal Biology
Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
DOI:

10:26 min

January 16, 2015

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Chapters

  • 00:05Title
  • 01:22Immature Mosquito Development and Survivorship Assays
  • 03:17Fecundity Assay by Administering Drug Through Artifitial Feeder to Female Mosquitoes
  • 05:56Biochemical Assay of Mosquito Enzyme Inhibition by Drug
  • 08:25Results: Effect of DZNep on Malaria Mosquitoes
  • 10:00Concluision

Summary

Automatic Translation

A protocol is developed to examine the effects of an epigenetic drug DZNep on the development, fecundity and survivorship of mosquitoes. Here we describe procedures for the aqueous exposure of DZNep to immature mosquitoes and a blood-based exposure of DZNep to adult mosquitoes in addition to measuring SAH hydrolase inhibition.

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