We present a method of preparing mica supported lipid bilayers for high resolution microscopy. Mica is transparent and flat on an atomic scale, but rarely used in imaging because of handling difficulties; our preparation results in even deposition of the mica sheet, and reduces the material used in bilayer preparation.
Matysik, A., Kraut, R. S. Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging. J. Vis. Exp. (88), e52054, doi:10.3791/52054 (2014).