13,397 Views
•
07:48 min
•
June 07, 2014
DOI:
Summary
Automatically generated
We present a method of preparing mica supported lipid bilayers for high resolution microscopy. Mica is transparent and flat on an atomic scale, but rarely used in imaging because of handling difficulties; our preparation results in even deposition of the mica sheet, and reduces the material used in bilayer preparation.
Related Videos
12:24
Supported Planar Bilayers for the Formation of Study of Immunological Synapses and Kinapse
Related Videos
12591 Views
07:56
Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
Related Videos
13700 Views
10:15
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Related Videos
14733 Views
09:38
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
Related Videos
14891 Views
20:00
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Related Videos
13896 Views
09:56
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
Related Videos
12384 Views
10:43
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Related Videos
2245 Views
12:18
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
Related Videos
3484 Views
11:55
Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
Related Videos
2170 Views
02:07
Planar Supported Lipid Bilayer Assay: A Microscopy-Based In Vitro Technique to Investigate Septin Assembly on Biological Membrane Mimics
Related Videos
234 Views
Read Article
Cite this Article
Matysik, A., Kraut, R. S. Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging. J. Vis. Exp. (88), e52054, doi:10.3791/52054 (2014).
Copy