/
/
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:27Isolation and Preparation of Primary Bone Macrophages from C57/B16 Mice
- 04:51Co‐incubation of Primary Murine Macrophages and C. neoformans
- 06:56Visualizing Non‐lytic Exocytosis in Real‐time Utilizing Digital Light Microscopy
- 08:11Results: Primary Murine Macrophages Undergo Different Types of Non‐lytic Exocytosis of C. neoformans
- 09:36Conclusion
We describe how to visualize macrophage-C. neoformans (Cn) interactions in real time, with specific emphasis on the process of non-lytic exocytosis using digital light microscopy. Using this technique individually infected macrophages can be studied to ascertain various aspects of this phenomenon.
