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December 21, 2014
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We describe a protocol to isolate and culture human amnion epithelial cells (hAECs) using animal product-free reagents in accordance with current good manufacturing practices (cGMP) guidelines.
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Cite this Article
Murphy, S. V., Kidyoor, A., Reid, T., Atala, A., Wallace, E. M., Lim, R. Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications. J. Vis. Exp. (94), e52085, doi:10.3791/52085 (2014).
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