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Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
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Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

DOI:
10.3791/52085-v

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05:31 min

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December 21, 2014

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, , , , ,
1Wake Forest Institute for Regenerative Medicine,Wake Forest University Health Sciences, 2The Ritchie Centre, Monash Institute of Medical Research,Monash University

Chapters

  • 00:05Title
  • 01:02Isolation of Amnion Epithelial Cells
  • 04:16Results: Representative hAEC Cultures
  • 05:08Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to isolate and culture human amnion epithelial cells (hAECs) using animal product-free reagents in accordance with current good manufacturing practices (cGMP) guidelines.

Tags

Human Amnion Epithelial CellsHAECsRegenerative MedicineMultipotent DifferentiationLow ImmunogenicityAnti-inflammatoryCell IsolationCryopreservationAnimal Product-freeCell TherapyClinical Applications

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