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November 18, 2014
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Summary
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MicroRNAs (miRNAs) are a widely conserved class of regulatory molecules. Here we describe a miRNA cloning method that relies upon two potent ligation steps followed by high-throughput sequencing. Our method permits accurate genome-wide quantitation of miRNAs.
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Cite this Article
Lee, J. E., Yi, R. Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing. J. Vis. Exp. (93), e52095, doi:10.3791/52095 (2014).
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