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Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

DOI:
10.3791/52107-v

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08:52 min

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February 03, 2015

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, , , ,
1MRC Centre for Inflammation Research, Queen’s Medical Research Institute,The University of Edinburgh, 2Department of Cell & Developmental Biology,University College London

Chapters

  • 00:05Title
  • 01:46Microinjection of Plasmid DNA
  • 04:16Conditional Skin Cell Transformation by 4-Hydroxytamoxifen (4-OHT)
  • 05:34Mounting and Live Imaging of Zebrafish Larvae
  • 07:06Results: Live Imaging of Innate Immune and Preneoplastic Cell Interactions in Larval Zebrafish Skin
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRASG12V expressing skin cells in zebrafish larvae.

Tags

Keywords: Live ImagingInnate Immune CellPreneoplastic CellZebrafish Skin4-Hydroxytamoxifen (4-OHT)KalTA4-ERT2/UAS Expression SystemHRASG12VTumorigenesisEctopic Gene Expression

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