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JoVE Journal
Immunology and Infection

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Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
 

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

Article DOI: 10.3791/52119
October 14th, 2014

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Summary October 14th, 2014

Mouse embryonic stem cells can be differentiated to T cells in vitro using the OP9-DL1 co-culture system. Success in this procedure requires careful attention to reagent/cell maintenance, and key technique sensitive steps. Here we discuss these critical parameters and provide a detailed protocol to encourage adoption of this technology.

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