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Immunology and Infection
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Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
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Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

DOI:
10.3791/52140-v

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10:03 min

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December 12, 2014

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,
1Inserm U1016,Institut Cochin, 2Cnrs, UMR8104, 3Université Paris Descartes,Sorbonne Paris Cité

Chapters

  • 00:05Title
  • 01:32Chemokine Stimulation
  • 02:41Cell Staining
  • 04:14Image Acquisition and Analysis
  • 07:23Results: Representative Chemokine-induced T Cell Polarization
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.

Tags

Keywords: Cell PolarizationChemokine SignalingImaging Flow CytometryActin CytoskeletonMHC Class IPhospho-ERMPar6/aPKC PathwayPharmacological Inhibition

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