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Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
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Immunology and Infection
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JoVE Journal Immunology and Infection
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

DOI:
10.3791/52184-v

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14:14 min

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December 06, 2014

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, , , , , ,
1CREA, Diagnostics Department,Spedali Civili di Brescia

Chapters

  • 00:05Title
  • 02:34Preparation of Triple-insert Plasmid
  • 04:49Standard Curve Preparation and Real Time PCR for Quantification of TRECS and KRECS
  • 07:25Calculations
  • 11:59Results: TREC and KREC Quantification in Healthy Individuals
  • 12:54Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

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Keywords: T-cell Receptor Excision Circles (TRECs)K-deleting Recombination Excision Circles (KRECs)Real-time PCRSimultaneous QuantificationImmune ReconstitutionBone Marrow TransplantationImmune DeficienciesImmunomodulating Drugs

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