Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress <em>In Vitro</em>

Marcus K. Giacci; Nathan S. Hart; Richard V. Hartz; Alan R. Harvey; Stuart I. Hodgetts; Melinda Fitzgerald

doi:10.3791/52221

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Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress <em>In Vitro</em>

JoVE Journal
Biology
Author Produced

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JoVE Journal Biology

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

8,711 Views

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08:16 min

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March 21, 2015

DOI:

10.3791/52221-v

DOI:

10.3791/52221-v

•

08:16 min

March 21, 2015

•

8704 Views

Marcus K. Giacci¹, Nathan S. Hart², Richard V. Hartz¹, Alan R. Harvey³, Stuart I. Hodgetts³, Melinda Fitzgerald¹

¹Experimental and Regenerative Neurosciences, School of Animal Biology,The University of Western Australia ²School of Animal Biology and The Oceans Institute,The University of Western Australia ³Experimental and Regenerative Neurosciences, School of Anatomy, Physiology and Human Biology,The University of Western Australia

Summary

Non-coherent Xenon light was passed through narrow-band interference and neutral density filters to deliver light of varying wavelength and intensity to cultured cells. This protocol was used to assess the effects of red/near-infrared light therapy on production of reactive species in vitro: no effects were observed using the tested parameters.