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JoVE Journal
Immunology and Infection
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Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
 

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Article DOI: 10.3791/52222
November 1st, 2014

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Summary November 1st, 2014

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

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