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Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
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Immunology and Infection
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JoVE Journal Immunology and Infection
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

DOI:
10.3791/52222-v

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07:31 min

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November 01, 2014

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, ,
1Biological Department,King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology,The University of Sheffield

Chapters

  • 00:00Title
  • 00:10Introduction
  • 01:14Animation
  • 02:37Washing
  • 03:22Antigen Challenge
  • 03:48Substrate
  • 04:23Stopping the Reaction
  • 04:47Reading
  • 04:54Calculations
  • 06:22Results
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

Tags

Keywords: Equine IgEFcεRIIn Vitro ModelRBL-2H3.1 CellsTransfectionAllergic Responseβ-hexosaminidase ReleaseDiagnostic ToolTherapeutic Intervention

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