Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor Fc&#949;RI

Sari Sabban; Hongtu Ye; Birgit Helm

doi:10.3791/52222

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Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Development of an <em>in vitro</em> model system for studying the interaction of <em>Equus</em> <em>caballus</em> IgE with its high-affinity receptor FcεRI

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

12,369 Views

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07:31 min

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November 01, 2014

DOI:

10.3791/52222-v

DOI:

10.3791/52222-v

•

07:31 min

November 01, 2014

•

1 Views

Sari Sabban¹, Hongtu Ye², Birgit Helm²

¹Biological Department,King Abdulaziz University, ²The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology,The University of Sheffield

Summary

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.