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Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
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Bioengineering
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JoVE Journal Bioengineering
Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

DOI:
10.3791/52224-v

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11:01 min

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September 25, 2016

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, , , , , , , , ,
1Radiology,University of Minnesota, 2Medicine,University of Minnesota, 3School of Public Health,University of Minnesota, 4Surgery,University of Arizona

Chapters

  • 00:05Title
  • 01:05Assemble the Continuous Feeding System
  • 04:30Spheroid Formation
  • 06:04Continuous Feeding Culture and Adjusted Feed Rate
  • 08:50Results: Improved Growth Curve and Cell Yields from Cells Grown in Stirred Suspension Bioreactors
  • 10:05Conclusion

Summary

Automatic Translation

Automatic Translation

Nutrient regulation using continuous growth adjusted feeding improves growth rates of mammalian cell spheroids compared to intermittent batch feeding for cultures in stirred suspension bioreactors. This study demonstrates the methods required for establishing simple adjusted rate fed cultures.

Tags

Keywords: Nutrient RegulationContinuous FeedingLarge-scale Cell ExpansionSpheroidsBioreactorPeristaltic PumpMedium ReservoirWaste ReservoirPerfusion TubingCell CultureCellular TherapyNutrient ControlGas ExchangeCell DensityCell YieldCell Loss

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