Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

Amy Weckle; Allison E. Aiello; Monica Uddin; Sandro Galea; Rebecca M. Coulborn; Richelo Soliven; Helen Meier; Derek E. Wildman

doi:10.3791/52227

Journal

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Medicine

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Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

JoVE Journal
Medicine

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JoVE Journal Medicine

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

16,043 Views

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11:31 min

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November 30, 2015

DOI:

10.3791/52227-v

DOI:

10.3791/52227-v

•

11:31 min

November 30, 2015

•

15999 Views

Amy Weckle^1,2, Allison E. Aiello³, Monica Uddin^1,4, Sandro Galea⁵, Rebecca M. Coulborn⁶, Richelo Soliven⁷, Helen Meier⁶, Derek E. Wildman^1,2

¹Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign ²Department of Molecular and Integrative Physiology,University of Illinois at Urbana-Champaign ³Department of Epidemiology, Gillings School of Global Public Health,University of North Carolina ⁴Department of Psychology,University of Illinois at Urbana-Champaign ⁵Department of Epidemiology, Mailman School of Public Health,Columbia University ⁶Department of Epidemiology,University of Michigan School of Public Health ⁷Center for Molecular Medicine and Genetics,Wayne State University School of Medicine

Summary

We outline a methodology for the processing of whole blood to obtain a variety of components for further analysis. We have optimized a streamlined protocol that enables rapid, high-throughput simultaneous processing of whole blood samples in a non-clinical setting.