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Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
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JoVE Journal Medicine
Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

DOI:
10.3791/52259-v

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08:39 min

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January 03, 2015

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, ,
1Department of Biomedicine,University Hospital Basel, 2Department of Internal Medicine II,University Hospital Tübingen

Chapters

  • 00:05Title
  • 01:35Cell Sorting and Plating
  • 04:16Serial Sphere Passaging
  • 05:59Results: Representative RFP+ and RFP- tumor Sphere Imaging
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

Tags

Keywords: Ovarian CarcinomaCancer Stem CellsCSCsSpheres AssayMulti Cell-based AssaysSingle Cell-based AssaysIn VitroSerum-free MediumGrowth FactorsSphere FormationMethylcellulose

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