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Undersöka spridning och toxicitet Prion-liknande proteiner Använda metazoan Model Organism<em> C. elegans</em
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Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
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Undersöka spridning och toxicitet Prion-liknande proteiner Använda metazoan Model Organism<em> C. elegans</em

DOI:
10.3791/52321-v

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12:57 min

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January 08, 2015

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, , , ,
1Department of Molecular Biosciences, Rice Institute for Biomedical Research,Northwestern University

Chapters

  • 00:05Title
  • 01:19Monitoring Transcellular Spreading of Prion-like Proteins by In Vivo Time-lapse Imaging
  • 03:31Genome-wide Screen for Suppression of Prion-induced Toxicity in C. elegans
  • 06:22Confirmation of Preliminary Screen Hits
  • 09:57Results: Analyzing the Spreading and Toxicity of Prion-like Proteins
  • 12:29Conclusion

Summary

Automatic Translation

Automatic Translation

Prion-like propagation of protein aggregates has recently emerged as being implicated in many neurodegenerative diseases. The goal of this protocol is to describe, how to use the nematode C. elegans as a model system to monitor protein spreading and to investigate prion-like phenomena.

Tags

Keywords: PrionPrion-like ProteinProtein AggregationProteostasisCaenorhabditis ElegansPrion DomainSup35Fluorescent TagGenetic ApproachesPrion PropagationCell-to-cell SpreadingProtein Misfolding DiseasesAlzheimer’s DiseaseParkinson’s DiseaseALSTransmissible Spongiform Encephalopathies

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