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Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
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JoVE Journal Chemistry
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

DOI:
10.3791/52323-v

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12:00 min

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January 22, 2015

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, , , , ,
1Department of Medicine,Brigham and Women’s Hospital and Harvard Medical School, 2Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care,Boston Children’s Hospital

Chapters

  • 00:05Title
  • 01:22Preparation of Stromal Cell-derived Factor-1α (SDF-1α) Glycan Nanoparticles
  • 03:34Measurement of Particle Size and Zeta Potential
  • 05:07Quantification of SDF-1α in the Particles
  • 06:26In Vitro Release Assay
  • 07:38Migration Assay
  • 09:19Results: Analysis of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
  • 11:23Conclusion

Summary

Automatic Translation

Automatic Translation

The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.

Tags

SDF-1αChitosanDextran SulfateNanoparticlesPolyelectrolyte ComplexesProtein DeliveryStem Cell Homing

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