Preparation and Characterization of SDF-1&#945;-Chitosan-Dextran Sulfate Nanoparticles

Andrew R. Bader; Tina Li; Weiping Wang; Daniel S. Kohane; Joseph Loscalzo; Ying-Yi Zhang

doi:10.3791/52323

Journal

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Chemistry

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Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

JoVE Journal
Chemistry

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JoVE Journal Chemistry

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

12,368 Views

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12:00 min

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January 22, 2015

DOI:

10.3791/52323-v

DOI:

10.3791/52323-v

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12:00 min

January 22, 2015

•

12352 Views

Andrew R. Bader¹, Tina Li¹, Weiping Wang², Daniel S. Kohane², Joseph Loscalzo¹, Ying-Yi Zhang¹

¹Department of Medicine,Brigham and Women’s Hospital and Harvard Medical School ²Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care,Boston Children’s Hospital

Summary

The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.