10,287 Views
•
12:13 min
•
April 05, 2015
DOI:
Summary
Automatically generated
Here we present a cost-effective method for defining chemical-genetic interactions in budding yeast. The approach is built on fundamental techniques in yeast molecular biology and is well suited for the mechanistic interrogation of small to medium collections of chemicals and other media environments.
Related Videos
10:43
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
Related Videos
11020 Views
07:55
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Related Videos
7936 Views
10:08
Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Related Videos
16829 Views
11:12
Identification of protein complexes with quantitative proteomics in S. cerevisiae
Related Videos
13147 Views
07:55
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Related Videos
1720 Views
05:44
Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
Related Videos
3492 Views
13:17
Microarray Analysis for Saccharomyces cerevisiae
Related Videos
13550 Views
11:59
Competitive Genomic Screens of Barcoded Yeast Libraries
Related Videos
18195 Views
10:57
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Related Videos
9682 Views
13:22
Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe
Related Videos
1717 Views
Read Article
Cite this Article
Dilworth, D., Nelson, C. J. Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae. J. Vis. Exp. (98), e52345, doi:10.3791/52345 (2015).
Copy