Journal
/
/
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl Channels and Transporters
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl<sup>–</sup> Channels and Transporters
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl Channels and Transporters

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl Channels and Transporters

9,819 Views

07:47 min

April 20, 2015

DOI:

07:47 min
April 20, 2015

9797 Views
,

Summary

Automatically generated

Proteoliposomes are used to study purified channels and transporters reconstituted in a well-defined biochemical environment. An experimental procedure to measure efflux mediated by these proteins is illustrated. The steps to prepare proteoliposomes, perform the recordings, and analyze data to quantitatively determine the functional properties of the reconstituted protein are described.

Related Videos

Read Article