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An Ex vivo Model to Study Hormone Action in the Human Breast
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JoVE Journal Medicine
An Ex vivo Model to Study Hormone Action in the Human Breast

An Ex vivo Model to Study Hormone Action in the Human Breast

DOI:
10.3791/52436-v

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12:31 min

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January 08, 2015

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, ,
1Swiss Institute for Experimental Cancer Research, School of Life Sciences,Ecole polytechnique fédérale de Lausanne

Chapters

  • 00:05Title
  • 03:05Tissue Digestion and Hormone Stimulation
  • 05:11Recovery of Tissue Microstructures
  • 06:38Samples Preparation for Flow Cytometry, Histology and Cell Culture
  • 09:47Results: Characterization of Tissue Microstructures
  • 11:20Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a novel ex vivo model to study hormone action in the human breast. It is based on tissue microstructures isolated from surgical breast tissue specimens which preserve tissue architecture, intercellular interactions, and paracrine signaling.

Tags

Ex Vivo ModelHuman BreastHormone ActionMammary Epithelial CellsHormone ReceptorBreast CancerDuctsLobulesStromal CellsRNA ExtractionProtein ExtractionHistological AnalysisFACS

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