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Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma
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Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma

Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma

DOI:
10.3791/52443-v

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10:58 min

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February 22, 2015

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, , , , ,
1Department of Neurosurgery,University of Michigan School of Medicine, 2Department of Pediatrics, Division of Hematology-Oncology,University of Michigan School of Medicine, 3Department of Cell and Developmental Biology,University of Michigan

Chapters

  • 00:05Title
  • 02:43Preparations for the Intra-ventricular Neonatal Injections
  • 04:15Intra-ventricula Neonatal Injections
  • 06:37Monitoring Plasmid Uptake and Tumor Formation and Progression
  • 08:17Results: Induced Glioblastoma Pathology
  • 09:54Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe an efficient and versatile protocol to induce, monitor and analyze novel glioblastomas (GBM) using transposon DNA injected into the ventricles of neonatal mice. Cells of the subventricular zone, which take up the plasmid, transform, proliferate and generate tumors with histo-pathological characteristics of human GBM.

Tags

Keywords: TransposonPlasmid DNASubventricular ZoneNeonatal MiceGlioblastomaSleeping Beauty Transposon SystemBioluminescenceHistologyImmunohistochemistryPrimary GBM Cell Lines

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