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Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode Caenorhabditis elegans
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Chapters
- 00:05Title
- 01:41Establishment of a Synchronized L1 Larvae Populations
- 03:40Establishment of a Synchronized L4 Larvae Populations
- 05:03Exposure of Worms to Chemicals in 96-well Plates
- 07:25Image Acquisition of Gravid Worm in a 384-well Plate
- 09:23Results: Germline Disruption is Detected by GFP Fluorescence in a C. elegans Reporter Strain Exposed to Nocodazole
- 10:19Conclusion
We describe the detailed steps of a high-throughput chemical assay in the nematode Caenorhabditis elegans used to assess germline toxicity. In this assay, disruption of germline function following chemical exposure is monitored using a fluorescent reporter specific to aneuploid embryos.
