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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
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JoVE Journal Engineering
Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

DOI:
10.3791/52456-v

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09:48 min

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February 27, 2015

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, , ,
1Physik-Department E22a,Technische Universität München, 2IMETUM,Technische Universität München

Chapters

  • 00:05Title
  • 01:25Tip Functionalization
  • 06:01Data Acquisition
  • 07:50Results: Single Molecule Adhesion of Polytyrosine on a Methyl-self-assembled Monolayer
  • 09:19Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to couple a large variety of single molecules covalently onto an AFM tip is presented. Procedures and examples to determine the adhesion force and free energy of these molecules on solid supports and bio-interfaces are provided.

Tags

Keywords: Atomic Force SpectroscopySingle Molecule AdhesionSingle Molecule AttachmentTip PassivationAdhesion ForceAdhesion LengthFree EnergyPolytyrosinePI-g-PSPOPESAMsSupported Lipid Bilayers

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