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JoVE Journal
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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
 

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Article DOI: 10.3791/52456
February 27th, 2015

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Summary February 27th, 2015

A protocol to couple a large variety of single molecules covalently onto an AFM tip is presented. Procedures and examples to determine the adhesion force and free energy of these molecules on solid supports and bio-interfaces are provided.

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