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1Department of Respiratory Medicine, Graduate School of Medicine,The University of Tokyo, 2Department of Clinical Laboratory, Graduate School of Medicine,The University of Tokyo, 3Division for Health Service Promotion,The University of Tokyo, 4Department of Biochemistry,Nihon University School of Dentistry, 5Department of Biochemistry,Ohu University School of Pharmaceutical Sciences
Chapters
- 00:05Title
- 01:51Primary Culture of Human Lung Fibroblasts
- 03:35Three-dimensional Co-culture of Human Lung Fibroblasts and Cancer Cells
- 06:27Air-liquid Interface Culture and Invasion Assay
- 07:14Results: Analysis of A549 Cells Co-cultured on a 3D Collagen Gel Embedded with Primary Cultures of Cancer-associated Fibroblasts
- 08:09Conclusion
Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.
