Three-dimensional Co-culture Model for Tumor-stromal Interaction

Masafumi Horie; Akira Saito; Yoko Yamaguchi; Mitsuhiro Ohshima; Takahide Nagase

doi:10.3791/52469

Journal

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Medicine

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Three-dimensional Co-culture Model for Tumor-stromal Interaction

JoVE Journal
Medicine

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JoVE Journal Medicine

Three-dimensional Co-culture Model for Tumor-stromal Interaction

17,341 Views

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08:39 min

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February 02, 2015

DOI:

10.3791/52469-v

DOI:

10.3791/52469-v

•

08:39 min

February 02, 2015

•

17316 Views

Masafumi Horie^1,2, Akira Saito^1,3, Yoko Yamaguchi⁴, Mitsuhiro Ohshima⁵, Takahide Nagase¹

¹Department of Respiratory Medicine, Graduate School of Medicine,The University of Tokyo ²Department of Clinical Laboratory, Graduate School of Medicine,The University of Tokyo ³Division for Health Service Promotion,The University of Tokyo ⁴Department of Biochemistry,Nihon University School of Dentistry ⁵Department of Biochemistry,Ohu University School of Pharmaceutical Sciences

Summary

Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.