Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

JoVE Journal Medicine
Three-dimensional Co-culture Model for Tumor-stromal Interaction
 

Three-dimensional Co-culture Model for Tumor-stromal Interaction

Article doi: 10.3791/52469
February 2nd, 2015 •
1,2, 1,3, 4, 5, 1
1Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, 2Department of Clinical Laboratory, Graduate School of Medicine, The University of Tokyo, 3Division for Health Service Promotion, The University of Tokyo, 4Department of Biochemistry, Nihon University School of Dentistry, 5Department of Biochemistry, Ohu University School of Pharmaceutical Sciences

Summary
Automatic Translation
February 2nd, 2015

Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter