Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

Hafsa Munir; G. Ed Rainger; Gerard B. Nash; Helen McGettrick

doi:10.3791/52480

Journal

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Immunology and Infection

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Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

10,181 Views

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11:30 min

•

January 07, 2015

DOI:

10.3791/52480-v

DOI:

10.3791/52480-v

•

11:30 min

January 07, 2015

•

6 Views

Hafsa Munir^1,2, G. Ed Rainger^1,2, Gerard B. Nash^1,2, Helen McGettrick^2,3

¹School of Clinical and Experimental Medicine,University of Birmingham, ²College of Medical and Dental Sciences,University of Birmingham, ³School of Immunity and Infection,University of Birmingham

Summary

The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.