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Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
JoVE Journal
Medicine
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JoVE Journal Medicine
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

DOI:
10.3791/52483-v

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06:33 min

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March 06, 2015

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, , , ,
1Department of Pediatric General and Thoracic Surgery,Cincinnati Children’s Hospital Medical Center, 2Department of Medicine, Section of Gastroenterology and Hepatology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 01:14Crypt Isolation from Whole Tissue
  • 03:31Crypt Culture in Basement Membrane Matrix
  • 04:27Results: Human Epithelial and Colonoids Cultured from Whole Tissue and Biopsy
  • 05:57Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.

Tags

Keywords: Human Epithelial EnteroidsHuman Epithelial ColonoidsIntestinal Stem CellsIntestinal EpitheliumGastrointestinal HomeostasisEx Vivo TechnologiesPrimary Cell Culture3D Epithelial OrganoidsCrypt-derived SystemBiopsy

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