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JoVE Journal
Medicine

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Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
 

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Article DOI: 10.3791/52483
March 6th, 2015

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Summary March 6th, 2015

We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.

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