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JoVE Journal
Developmental Biology

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Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
 

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Article DOI: 10.3791/52496-v 09:22 min February 9th, 2015
February 9th, 2015

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Summary

We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.

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Keywords: Xenopus Laevis Oocytes In Vitro Maturation Intracytoplasmic Sperm Injection ICSI Embryonic Development Maternal Factors Gene Interference Antisense Oligonucleotides MRNA Injection Protein Expression Swimming Tadpole
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