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JoVE Journal
Developmental Biology

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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
 

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Article DOI: 10.3791/52539-v 14:46 min May 28th, 2015
May 28th, 2015

Chapters

Summary

Genome editing tools such as the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system have greatly improved gene targeting efficiency in human induced pluripotent stem cells (hiPSCs). This manuscript describes a protocol for generating lineage specific hiPSC reporter using CRISPR/Cas system assisted homologous recombination.

Tags

Keywords: HiPSC Neural Lineage Gene Targeting CRISPR/Cas9 Cas9 Double Nickase Homologous Recombination Reporter Lines Genetic Manipulation Genome Editing
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