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Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
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Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

DOI:
10.3791/52542-v

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08:25 min

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March 23, 2015

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, , , , ,
1Department of Cell and Molecular Physiology,Loyola University Chicago, 2Department of Biomedical Engineering,University of Alabama at Birmingham, 3Department of Bioengineering,Clemson University

Chapters

  • 00:05Title
  • 01:45Seeding and Culturing of HL-1 Atrial Myocytes on Coverslips
  • 04:12Loading HL-1 Cells with Calcium- and Voltage-sensitive Dyes
  • 05:20Dual Channel Optical Mapping of HL-1 Cells
  • 06:42Results: Action Potentials and Calcium Signals Propagate Across a Monolayer of HL-1 Atrial Myocytes
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes the technique used to perform dual channel optical mapping in cultured HL-1 atrial cell monolayers. This unique protocol allows the simultaneous visualization of both calcium (Ca) and voltage (Vm) activity in the same area for the detailed detection and analysis of electrophysiological properties of culture monolayers.

Tags

Keywords: Optical MappingHL-1 CellsAction PotentialsCalcium TransientsDual ChannelCardiac PhysiologyArrhythmia

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