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Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens

Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens

DOI:
10.3791/52552-v

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06:57 min

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February 20, 2015

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, , ,
1Department of Pathology and Pathogen Biology,Royal Veterinary College, London, 2BIOPHARM,Research Institute of Biopharmacy and Veterinary Drugs

Chapters

  • 00:05Title
  • 01:16Template Preparation
  • 02:44Eimeria LAMP Primer Preparation
  • 03:50Eimeria LAMP Assay and Read-out
  • 05:22Results: Eimeria LAMP Assay Testing
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.

Tags

EimeriaCoccidiosisLAMP AssaySpecies-specific DetectionChickenGenomic DNAMolecular Diagnosis

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