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Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy
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Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

DOI:
10.3791/52577-v

•

06:51 min

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February 01, 2015

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1LOEWE Excellence Cluster for Integrative Fungal Research (IPF),Senckenberg Research Institute

Chapters

  • 00:05Title
  • 01:06P. chrysogenum Preparation
  • 02:16Starvation Pad Cultivation Preparation
  • 03:22P. chrysogenum Starvation Pad Inoculation and Incubation
  • 04:25Microscopic Sample Analysis
  • 05:11Results: Representative GFP-SKL Localization
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

A convenient and powerful method for studying autophagy in Penicillium chrysogenum by using starvation pads (mixtures of agarose and tap water in a microscope slide containing a central cavity) is presented here.

Tags

Keywords: AutophagyPenicillium ChrysogenumStarvation PadsFluorescence MicroscopyCellular Quality ControlOrganelle DynamicsProtease ActivityFilamentous FungusMicroscopy Evaluation

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