15,779 Views
•
06:31 min
•
May 20, 2015
DOI:
Summary
Automatically generated
The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.
Related Videos
04:28
Lung Tumor Cell Recruitment Assay
Related Videos
5244 Views
08:28
Experimental Metastasis Assay
Related Videos
20225 Views
07:44
Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
Related Videos
10033 Views
07:39
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
Related Videos
9184 Views
08:02
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
Related Videos
5472 Views
08:01
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
Related Videos
1812 Views
10:15
Isolation and Characterization of Neutrophils with Anti-Tumor Properties
Related Videos
24769 Views
11:19
Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
Related Videos
12329 Views
N/A
Isolation of Immune Cells from Primary Tumors
Related Videos
37389 Views
11:57
Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
Related Videos
10155 Views
Read Article
Cite this Article
Dong, S., Maziveyi, M., Alahari, S. K. Primary Tumor and MEF Cell Isolation to Study Lung Metastasis. J. Vis. Exp. (99), e52609, doi:10.3791/52609 (2015).
Copy