The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.
Dong, S., Maziveyi, M., Alahari, S. K. Primary Tumor and MEF Cell Isolation to Study Lung Metastasis. J. Vis. Exp. (99), e52609, doi:10.3791/52609 (2015).