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Dried Blood Spots – Preparing and Processing for Use in Immunoassays and in Molecular Techniques
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Dried Blood Spots – Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots – Preparing and Processing for Use in Immunoassays and in Molecular Techniques

DOI:
10.3791/52619-v

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11:28 min

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March 13, 2015

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, ,
1Institute of Virology, National Reference Centre for Hepatitis C, Essen University Hospital,University of Duisburg-Essen

Chapters

  • 00:05Title
  • 02:43Collection of Blood and Preparation of Blood Spots from Skin Puncture
  • 04:38Drying and Storage of Dried Blood Spots
  • 05:40Elution of Dried Blood Spots and Analysis of Eluates
  • 07:39Results: Testing of Dried Blood Spot Eluates for Markers of Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), and Human Immunodeficiency Virus (HIV) Infections
  • 10:11Conclusion

Summary

Automatic Translation

Automatic Translation

The preparing and processing of dried blood spots (DBS) for their final analysis are still poorly standardized for most diagnostic applications. To overcome this shortcoming, a comprehensive step-by-step protocol is suggested and subsequently evaluated with regard to its effectiveness for detecting markers of viral infections.

Tags

Keywords: Dried Blood SpotsDBSImmunoassaysMolecular TechniquesDiagnosticInfectious DiseasesNewborn ScreeningPre-analytical VariablesSample CollectionSample PreparationSample DryingSample StorageSample TransportationSample ElutionHepatitis BHepatitis CHIVAutomated Analytical Platform

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